The effect of Rhodamine B on the cerebellum and brainstem tissue of Rattus norvegicus

  • Dewi Ratna Sulistina
    Doctoral Program of Public Health, Faculty of Public Health, Universitas Airlangga, Mulyorejo, Surabaya, Indonesia.
  • Santi Martini
    Department of Epidemiology, Faculty of Public Health, Universitas Airlangga, Mulyorejo, Surabaya,, Indonesia.


Background: Rhodamine B is a component of xenobiotic substance metabolized by cytochrome P450 in the body to produce free radicals, which affects the activity of Superoxidase Dismutase (SOD), thereby, leading to oxidative stress, injury, increase in cell apoptosis and brainstem. This study aims to determine the effect of Rhodamine B on BAX and BCL-2 in the cerebellum and brainstem tissue of Rattus norvegicus.
Design and Methods: The True Experimental Design was used to carry out a post-test examination on the control group of twenty-eight Wistar female Rattus norvegicus mice between the ages of 10-12 weeks. Then, samples were categorized into 4 groups in body weight doses of 4.5 mg/200g, 9 mg/200g, and 18 mg/200g. They were administrated with Rhodamine B personde for 36 days.
Results: The results showed that Rhodamine B had a direct and indirect effect on BAX and BCL-2 expressions, respectively, in the cerebellum tissue and wistar strain of Rattus norvegicus. In addition, the positive path coefficient of BAX expression has a positive effect on BCL-2. This means that an increase in BAX has a direct impact on decreasing BCL-2 expression in cerebellum tissue and brainstem of Rattus norvegicus wistar strain along with an increased dose of Rhodamine B.
Conclusions: In conclusion, Rhodamine B tends to increase BAX expression which directly decreases BCL-2 in Cerebellum tissue and Brainstem in Rattus norvegicus along with increasing doses.